ATUMTOME System

ARRAY TOMOGRAPHY

Automated Tape Collecting Ultramicrotome System

Description

  • Developed at Harvard University, where Professor Jeff Lichtman and his team designed the instrument
    ( brain’s neural pathways )
  • RMC ATUMtome is a unique system for collecting sections on a continuous tape.
  • Summary procedure
    • Resin-embedded specimen is used on the ultramicrotome to cut serial sections
    • Automatically removing the serial sections from the water surface in sequence
    • Tape is mounted onto a silicon wafer substrate or glass slides
    • Ready for imaging with a scanning electron microscope or via other microscopy techniques

              Benefits

              • Do not lose one single section – collect hundreds to thousands of sections on a continuous tape, uninterrupted
              • Non-destructive preparation – sections can be reviewed many times at varying magnification.Use low magnification for locating regions of interest, then zoom in for higher resolution imaging.This saves time, focusing only on your regions of interest
              • Sections available for years into the future for processing, post-staining, immunogold labeling and correlative imaging.
              • Determine sample viability early on in the process, so you don’t waste valuable time cutting and imaging a problem sample. Allows correlative microscopy for localization and then ultrastructural imaging.
              • Time saving – short pixel dwell time for fast detection during SEM imaging.
              • Uses standard sample preparation techniques and resins.
              • Various Kapton tape coatings available to prevent section charging.
              • Extremely cost effective method of 3D imaging, from hundreds to thousands of sections.
              • Can be upgraded to the PT3D, featuring 1 mm of uninterrupted sectioning.

                          INCLUDES

                          • ATUM continuous tape feed mechanism with PC touchscreen control
                          • PowerTome PTPCZ with HD Video Package
                          • Air-activated anti-vibration table with Compressor and Ergonomic lab chair
                          • Diatome Ultra Maxi Diamond Knife, 4mm, 35° or 45°
                          • Start-up supply of Kapton tape
                          • 4” diameter silicon wafers
                          • Environmental chamber
                          • Anti-static device
                          • Water level control system
                          • Wafer workstation